51. ワタの生産する根寄生雑草発芽刺激物質

概要

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Seed germination of Orobanche spp. and Striga spp. is triggered by germination stimulants (strigolactones) released from roots of host plants. We have established an LC/MS/MS method for determination and quantification of strigolactones (Sato et al., 2003). This method enables direct analysis of strigolactones in crude ethyl acetate extracts of root exudates without any purification. By using this LC/MS/MS method, we identified and quantified strigolactones in root exudates of several plant species. In this study, we aimed to clarify strigolactones produced by cotton. In our experiment, cotton was confirmed to produce strigol and strigyl acetate but not orobanchol or alectrol. Time-course of strigol production by cotton seedlings was examined and higher strigol production was observed for 5-7 days after incubation. Cotton seedlings were found to produce 30 pg/day/plant of strigol and 3 pg/day/plant of strigyl acetate. Strigyl acetate was only 1/100 as active as strigol on O. minor seed germination. Characterization of strigolactones produced by other host plants is in progress.
植物化学調節学会の論文
2003-10-10