25.ヒメツリガネゴケ野生株およびCCD8破壊株(Ppccd8)の茎葉体が生産するストリゴラクトンの解析(口頭発表)

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At the previous meeting, we reported that chloronema of Physcomitrella patens subsp. patens produces strigolactones (SLs). In this study, WT and the CCD8 loss-of-function mutant (Ppccd8) of P. patens were grown in liquid BCD culture for about 30 days to obtain gametophores during which the media were replaced with fresh one weekly. Then, they were grown for additional 3 days and media collected were extracted with ethyl acetate and SLs in the extracts were analyzed by LC-MS/MS. Both WT and Ppccd8 were found to produce 7-oxoorobanchyl acetate and strigol at similar levels, while fabacyl acetate, orobanchyl acetate, 7α-hydroxyorobanchyl acetate and orobanchol were produced only by WT. Although there is no MAX1 homolog in the moss genome, P. patens produces SLs, suggesting that MAX1 functions in later steps of the active branching inhibiting hormone biosynthesis, i.e, conversion of SLs to the branching inhibitor. Alternatively the moss may have other P450(s) which replace the function of MAX1 for SL biosynthesis.
2010-10-01