45. 非光合成植物の光センシング系はどの程度保存されているか? : ヤセウツボフィトクロム遺伝子の発現および局在解析

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Light is the most important environmental signal for plants. Plants have elaborate systems to detect the light-environment and adapt to the changes. Lights are involved in every aspects of plant growth. Only three families of photoreceptor are known to date. And the signaling cascades seem to form complicated network. We focused attention on non-photosynthetic plants to obtain new and important information on the light sensing systems in plants. Those non-photosynthetic plants lost their photosynthetic ability evolutionary after they had gained the mechanism to obtain their nutrition from other sources. We assumed that basic photomor-phological responses such as germination and flowering still remain but photo-signaling pathways to regulate the photosynthesis change from those of photosynthetic plants. And the domains only related to the photoregulation in the photoreceptors also may altered. Based on the hypothesis, we cloned PHYA homologs from non-photosynthetic plants and found that there are many substituted or deleted amino acids in regions where are conserved in the PHYA sequences from photosynthetic plants. In this study, we analyzed expression and subcellular localization of PHYA from Orobanche minor (OmPHYA) to investigate their function. The expression levels of OmPHYA were quantified by real time PCR in O. minor grown in the light and dark conditions. It is shown that the expression level of OmPHYA was more significant in O. minor grown in the dark. The vector was constructed to express OmPHYA and GFP fused protein and the gene was expressed in the protoplasts of Arabidopsis thaliana by the PEG method. As results, control PHYA of A. tahaliana localized cytoplasm in dark and nucleus in light but OmPHYA localized cytoplasm in dark and chloroplasts in light.
植物化学調節学会の論文
2003-10-10

45. 非光合成植物の光センシング系はどの程度保存されているか? : ヤセウツボフィトクロム遺伝子の発現および局在解析

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