31. アブシジン酸代謝阻害剤の探索

概要

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Abscisic acid (ABA) catabolism in plant is predominantly regulated by ABA 8'-hydroxylase, which is a cytochrome P450. Among the known cytochrome P450 inhibitors with triazole group we tested, uniconazole-P inhibited ABA catabolism in cultured tobacco bright yellow-2 cells. On the basis of a structure-activity relationship study of uniconazole, we found that diniconazole, which is known as a fungicide, was more effective ABA catabolic inhibitor than uniconazole-P. Diniconazole showed the potent inhibitory activity to CYP707A3, Arabidopsis ABA 8'-hydroxylase, in in vitro assay. Diniconazole-treated plants kept higher ABA contents and higher transcription levels of ABA response genes during rehydration than those of non-treatmented. These results strongly suggest that ABA catabolic inhibitors targeting ABA 8'-hydroxylase can regulated the ABA content in plants. Moreover, we performed optical resolution of diniconazole and showed that S-form of diniconazole, a less active isomer as fungicide, was more active isomer as ABA catabolic inhibitor than R-form.
植物化学調節学会の論文
2004-10-13