53. イネにおけるエリシター応答性WRKYの病害抵抗性への関与

概要

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WRKY factors are a large family of plant-specific transcription factors that specifically bind to the W-box elements [(T)TGAC(C/T)], and have been suggested to have regulatory functions in response to pathogen infection and other stresses. We isolated the cDNAs encoding two chitin elicitor-induced WRKY genes, OsWRKY53 and OsWRKY71, from suspension-cultured rice cells. Expression of the OsWRKY53 and OsWRKY71 genes were induced by a sphingolipid elicitor as well as a chitin elicitor in suspension-cultured rice cells. The expression of these genes were also induced in rice plants infected with the fungal pathogen Magnaporthe grisea. Fusions of OsWRKY53 and OsWRKY71 with Green Fluorescent Protein were detected exclusively in the nuclei of onion epidermal cells. By means of gel mobility-shift assay, we confirmed that these WRKY proteins could specifically bind to the W-box motifs in the 5' -upstream regions of diterpene cyclases involved in rice diterpenoid phytoalexin biosynthesis that is induced by the elicitors. These evidences strongly suggest that OsWRKY53 and OsWRKY71 encode transcription factors involved in defense responses against infection with pathogens in rice. To elucidate the roles of OsWRKY53 and OsWRKY71, the survey of target genes of both WRKY factors is also under way.
植物化学調節学会の論文
2004-10-13