23. イネにおけるエリシター応答性WRKY型転写因子OsWRKY53、OsWRKY71の機能解析

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WRKY proteins are a large family of plant-specific transcription factors that specifically bind to the W-box elements. Many WRKY proteins have been suggested to have regulatory functions in the response to pathogen infection and other stresses. In suspension-cultured rice cells, PR proteins and phytoalexins are produced in response to exogenously applied elicitors from the pathogen Magnaporthe grisea. By means of microarray analysis, we isolated the cDNAs encoding two chitin elicitor-induced WRKY genes, OsWRKY53 and OsWRKY71, from suspension-cultured rice cells. Expression of these genes were induced by a sphingolipid elicitors as well as a chitin elicitor in suspension-cultured rice cells, and were induced in rice plants infected with M. grisea. By means of gel mobility-shift assay, we confirmed that these WRKY proteins could specifically bind to the W-box motifs. The survey of target genes of OsWRKY53 and OsWRKY71 was performed by microarray analysis using OsWRKY53 or OsWRKY71-overexpressing mutants. As the results of microarray analysis, several defense-related genes such as PR protein genes and WRKY genes were upregulated, and the presence of W-boxes was observed in the 5' upstream regions of the upregulated genes. Moreover, the expression of these defense related genes were induced by a chitin elicitor in suspension-cultured rice cells. These results strongly suggest that OsWRKY53 and OsWRKY71 are involved in elicitor-induced defense responses in rice.
植物化学調節学会の論文
2005-10-13

23. イネにおけるエリシター応答性WRKY型転写因子OsWRKY53、OsWRKY71の機能解析

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