22. イネのファイトアレキシン生合成酵素遺伝子クラスターの機能解析

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Diterpenoid phytoalexins such as momilactones and (-)-phytocassanes are produced in suspension-cultured rice cells treated with a chitin elicitor and in the rice plant treated with UV irradiation. The diterpenoid phytoalexins are biosynthesized from geranylgeranyl diphosphate via ent- or syn-copalyl diphosphate (CDP) and diterpene hydrocarbons. Most of biosynthetic steps from the diterpene hydrocarbons into respective diterpenoid phytoalexins are considered to be catalyzed by P450 enzymes. However, functional analysis of the P450 enzymes involved diterpenoid phytoalexins has not been reported. We found that there are six P450 genes, ent-CDP synthase gene (OsCyc2), and ent-cassa-12, 15-diene synthase gene (OsDTC1) in a 215 kb region on chromosome 2, and that there are two P450 genes, syn-CDP synthase gene (OsCyc1), and 9βH-pimara-7, 15-diene synthase gene (OsKS4) in a 155 kb region on chromosome 4. Expression of the above eight P450 genes on chromosomes 2 and 4 were induced in suspension-cultured rice cells treated with a chitin elicitor and/or in the rice plants irradiated UV light. RNAi of the two P450 on chromosome 4 resulted in suppression of production of momilactones, suggesting that these two P450 genes in addition to OsCyc1 and OsKS4 are involved in momilactone biosynthesis. We also detected a chitin elicitor-inducible dehydrogenase gene in the 155 kb gene cluster on chromosome 4 that might encode momilactone A synthase.
植物化学調節学会の論文
2005-10-13

22. イネのファイトアレキシン生合成酵素遺伝子クラスターの機能解析

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