86 光発芽レタス種子におけるジベレリン生合成初期段階を触媒する酵素遺伝子のクローニング

概要

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Germination of lettuce (Lactuca sativa L. cv. Grand Rapids) seeds is regulated by phytochrome, which is mediated by gibberellin (GA) biosynthesis. In order to investigate which step of GA biosynthesis is regulated by phytochome, 4 cDNAs encoding enzymes catalyzing later steps of GA biosynthesis were cloned in our previous study; Ls20ox1,Ls20ox2,Ls3h1,Ls3h2,2-oxoglutarate-dependent dioxigenases genes. Furthermore, northern blot analysis suggested that Ls3h1 gene expression is regulated by phytochrome. In this study, we tried to isolate cDNAs encoding enzymes catalyzing early steps of GA biosynthesis, two cyclases, copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), and two P450 monooxigenases, ent-kaurene oxidase (KO), ent-kaurenoic acid oxidase (KAO). By RT-PCR using degenerate primers, cDNAs encoding these 4 enzymes were successfully isolated from the lettuce seeds; LsCPS1,LsKS1,LsKO1,LsKO2,LsKAO1. We will show the results of expression analysis of these genes during germination of the lettuce seeds.
植物化学調節学会の論文
2002-10-31