48.乾燥応答におけるABA生合成酵素AtNCED3の発現調節(口頭発表)

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Abscisic acid (ABA) is an essential hormone for plant's adaptation to drought stress. InArabidopsis, induction of9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3) mRNA accumulation is the regulatory step in ABA biosynthesis under drought stress conditions. However, regulatory mechanisms underlying drought induction of AtNCED3 is largely unknown. In the present study, we investigated the expression and localization of AtNCEDS in turgid and dehydrated plants using the specific antibody. Western blotting analysis showed that AtNCED3 was undetectable in turgid plants. Accumulation of a 64 kDa protein corresponding to the AtNCEDS was observed after 30 min of dehydration. After 1 hour of dehydration, two bands (64 kDa and 56 kDa) were detected. By chloroplast fraction experiment, 64 kDa form was found in thylakoid membrane fraction and the other form was in stroma. During rehydration, 64 kDa form diminished faster than 54 kDa form, which was correlated with the change in ABA levels. These results suggested that 64 kDa form is the active form. In situ hybridization experiment showed that drought induction of AtNCEDS was detected in the vascular bundles. Immunohistochemical analysis also confirmed that AtNCED3 was localized in the vascular parenchyma cells under dehydration condition. In addition, AtABA2 and AA03 were also localized in the identical tissues. These results indicate that drought induced ABA production occurs in vascular systems.
植物化学調節学会の論文
2007-10-05

48.乾燥応答におけるABA生合成酵素AtNCED3の発現調節(口頭発表)

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