43.タイリングアレイを用いたシロイヌナズナの種子におけるトランスクリプトーム解析(口頭発表)

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The phytohormone abscisic acid (ABA) plays an important role for seed dormancy. Indeed, ABA-deficient mutant, aba2, shows reduced seed dormancy, whereas ABA-over-accumulation mutant, cyp707a1 cyp707a2 cyp707a3 triple mutant, exhibits strong seed dormancy. Thus, ABA regulates a large number of genes that is involved in seed dormancy. To explore genome-wide expression patterns and regulatory mechanisms in Arabidopsis seeds, comprehensive expression analysis was performed using whole-genome tiling array. Although there was large difference in the endogenous ABA levels between aba2, and cyp707a triple mutant dry seeds, a large number of genes was not drastically changed in these mutants, indicating that the difference of endogenous ABA levels in dry seeds does not influence global gene expression. In contrast to dry seeds, the transcript levels of many genes in both mutants were drastically changed compared to those of wild type. Transcriptome analysis identified 380 ABA-upregulated and 540 ABA-downregulated AGI code genes at 24 h after imbibition. Moreover, whole-genome transcriptome analysis identified 4884 non-redundant new transcription units (TUs) in Arabidopsis seeds. Among new TUs, 4559 TUs probably encoded hypothetical non-coding RNAs (ncRNAs). We identified 127 ABA-upregulated and 13 ABA-downregulated new TUs at 24 h after imbibition. Furthermore, 26 ncRNAs were conserved in either rice or poplar. At present, we're proceeding analysis of functional ncRNA.
植物化学調節学会の論文
2007-10-05

43.タイリングアレイを用いたシロイヌナズナの種子におけるトランスクリプトーム解析(口頭発表)

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