LC-ESI-MS/MSによる植物ホルモンの一斉分析

概要

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Plant hormones are signal compounds, which regulate various responses at a low concentration in plants. Endogenous levels of them had been quantified mainly by using GC-EI-MS. Recently, microanalysis by using LC-ESI-MS/MS has been carried out frequently. This method does not require complicated purification and derivatization that GC-EI-MS requires. However, because the ionization efficiency in LC-ESI-MS/MS method is susceptible to impurities, this requires purification suitable to this system. In RIKEN Plant Science Center, we aim to establish a system for comprehensive analysis of all bioactive plant hormones and their precursors/metabolites. Here, we report our current method for purification and quantification of bioactive hormones (GA_1, GA_4, ABA, IAA, JA, SA, CKs and BRs) in dry seeds, leaves and flowers of Arabidopsis.
植物化学調節学会の論文
2007-12-20