52. 植物培養細胞をもちいたストリゴラクトン生合成経路の解析(口頭発表,植物化学調節学会第46回大会)

概要

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Strigolactones (SLs) are host recognition signals for root parasitic plants and arbuscular mycorrhizal fungi in the rhizosphere. In addition, shoot branching is inhibited by SLs in host plants. It has been demonstrated that SLs are derived from the carotenoid pathway in plants. However, the biosynthesis pathway of SLs has not been fully elucidated by genetic approaches. In order to propose a possible biosynthesis pathway of SLs in plants we investigated SL metabolisms by a feeding experiment using plant cell cultures. We found that plant cultured cell lines of rice and Arabidopsis produce SLs at higher levels than in plants and examined an optimum condition for the SL feeding experiment. Feeding experiments were carried out using the Oc strain, a suspension cell culture of rice provided from RIKEN BioResource Center of Japan, with deuterium-labeled SLs. Deuterium-labeled 5-deoxystrigol and 2'-epi-5-deoxystrigol were converted into several monohydroxylated metabolites in rice Oc cells. These metabolites were not identical with known monohydroxylated SLs such as strigol, orobanchol and sorgomol, but some of them had a germination-stimulant activity in bioassay using Orobanche seeds.
2011-10-03