38. 誘導体化によるLC-ESI-MS/MSを用いたジベレリン分析の高感度化(口頭発表,植物化学調節学会第46回大会)

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Gibberellin (GA) are plant hormone that regulates plant growth and environmental responses. We had already reported the method for GA analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Recently, plant hormone researches require tissue/organ specific high sensitive quantification analysis. In this meeting, we will report a derivatization method for high sensitive gibberellin analysis by LC-ESI-MS/MS. In addition to previously reported GA_1, GA_4, GA_8, GA_9, GA_<19>, GA_<20>, GA_<24>, GA_<44> and GA_<53>, kaurenoic acid, GA_<15>, GA_<29>, GA_<34> and GA_<51> were derivatized by 4% of 0.5 M 2-bromoethyltrimethylammonium bromide 70% acetonitrile solution in 75% 1-propanol, 20% water and 1% triethylamine. In case of 20 μl of reaction volume in capped glass capillary, 45 to 85% of GA had been reacted in 2 hours. In case of 100 μl of reaction volume in screw capped glass vial, approximately 30% of GA had been reacted in 3 hours. These results suggest that concentration affects reaction efficiency in this derivatization method. Detected areas of derivatized GA were from similar to 10 times larger than original GA. One product ion was determined in all GA. Acidic fraction of Arabidopsis leaves was prepared by hydrophobic, strong cation exchange and weak anion exchange extraction. As a result of derivatization, more than 25 times larger areas were determined in both GA_1 and GA_4 but higher base lines gave similar S/N compare to original GA analysis method.
2011-10-03

38. 誘導体化によるLC-ESI-MS/MSを用いたジベレリン分析の高感度化(口頭発表,植物化学調節学会第46回大会)

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