32.新奇ジベレリン信号伝達因子GAF1の機能解析(口頭発表)

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Gibberellins regulate germination, elongation and flowering. GAI proteins are members of the plant-specific GRAS protein family and act as repressors of the GA signaling pathway. GAI proteins are rapidly degraded in the presence of GA. The degradation mechanism is beginning to be uncovered by the discovery of GA receptor and F-box protein, but the downstream signaling of GAI proteins is unknown. We have identified a GAI interacting protein, named GAF1, using a modified yeast two hybrid screening method that we developed. GAF1 is a novel transcriptional factor that binds to DNA in a sequence-specific manner. Arabidopsis plants that overexpress GAF1 are early flowering with larger leaves. Our trans-activation assay in yeast and plant suggests that GAI protein regulates the function of GAF1 protein.
2008-10-06