74.トウモロコシ幼葉鞘先端におけるindole-3-acetic acid合成に影響を与える化合物のケミカルスクリーニング(口頭発表)

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The plant hormone, auxin (IAA) plays important roles in many plant physiological events. Recently, genetic approaches have clarified several aspects of the IAA-regulated network, including IAA transport and IAA signal transduction; however, the mechanisms of IAA biosynthesis remain largely uncharacterized. We have previously shown that maize coleoptile 2-mm tips can synthesize IAA from tryptophan and the synthesized IAA is immediately transported basipetaly. In recent work, the Chemical Genomics has been reported as a powerful approach to dissect processes that may be intractable using conventional genetics because of gene lethality or redundancy. Here, by using this system, we have developed a screen for identifying small molecule affecting IAA biosynthesis in maize coleoptile tips. The small-molecules library (HitFinder) was purchased from Maybridge. (1) At first, we have checked the effect of chemicals on gravitropic curvature. The effects of chemicals on the degrees of curvature of coleoptiles were compared to those of K-phosphate buffer (KPB) and NPA in KPB treatments as positive and negative controls, respectively. (2) Second, the effect of chemicals on the rate of IAA synthesis has been investigated. The amounts of IAA were detected using HPLC-fluorescent photometer. Here, we will report about the detail of the method of the chemical screening and the chemicals that we identified.
植物化学調節学会の論文
2008-10-06