35.シロイヌナズナ切断花茎の組織癒合に関与する植物ホルモンシグナリングと転写因子(口頭発表)

概要

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Arabidopsis cut flowering stems were analyzed to elucidate its molecular mechanism. Microarray analysis demonstrated differential gene expression between cut and non-cut flowering stems and their changes in expression during reunion process. Genes determined to be up-regulated in the reunion process include those involved in cell division, cell wall modification, phytohormone-related gene and transcription factors (TF). To understand how phytohormone involves in the tissue-reunion process, we focused on the phytohormone related genes and putative downstream TFs. Tissue-reunion was inhibited by decapitation or application of TIBA, and pin1 mutant, in which auxin transport was suppressed, showed lower efficiency in the reunion process. Gene expression analysis showed that AP2- and NAC-type TFs were upregulated at 1 and 3 days after cutting and the expression was depend on jasmonic acid (JA) and auxin, respectively. The gene-suppressing transformants for AP2-TF or NAC-TF using CRES-T method did not show normal reunion process. Ethylene-signaling deficient mutant also showed lower efficiency in tissue-reunion and gene expression analysis showed that AP2-TF and NAC-TF were also ethylene-regulated. These results suggest that JA/auxin/ethylene signaling pathways and putative downstream TFs have an important role in the regulation of gene expression during tissue-reunion of Arabidopsis cut flowering stems.
2010-10-01