15 キュウリ・トマト切断胚軸における皮層の組織癒合に対する子葉およびジベレリンの関与

概要

論文の詳細を見る
Cucumber hypocotyls were cut in half transversely, and morphological and histochemical analyses of the process of tissue-reunion in the cortex were performed. Cell division in the cortex commenced 3 days after cutting, and the cortex was nearly fully united within 7 days. DAPI- staining and BrdU-labeling experiments indicate that nuclear DNA synthesis occurred during this process. A specific accumulation of pectic substances was observed in the cell wall of attached cells in the reunion region of the cortex. Cell division in the tissue-reunion process in the cortex was strongly inhibited by removal of the cotyledon, and this inhibition was reversed by the application of gibberellin (10^<-4> M GA_3), but not auxin (10^<-4> MIAA), to the tip of the cotyledon-excised plant. Furthermore, cell division in the cortex was inhibited by treatment of the cotyledon with 10^<-4>M uniconazole-P, and this inhibition was also reversed by simultaneous application of GA. In contrast, removing the shoot apex did not inhibit tissue-reunion, and normal cortex tissue-reunion was observed when 10^<-4>M TIBA was applied to the upper hypocotyl above the position of the cut. Furthermore, similar phenomena were observed in tomato seedlings, and cell division in the tissue-reunion process did not occur in cut hypocotyls of a tomato GA-deficient mutant (gib-1). These results suggest novel functions for GA in cell division during the process of tissue-reunion in the cortex, as well as involvement of the cotyledon in the production of GA.
植物化学調節学会の論文
2001-10-09

15 キュウリ・トマト切断胚軸における皮層の組織癒合に対する子葉およびジベレリンの関与

スポンサーリンク

概要

著者

関連論文

スポンサーリンク