57.ブラシノライド生合成酵素CYP85Aファミリーの機能解析 : バイヤー・ビリガー酸化に必要な配列上の領域(口頭発表)

概要

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Brassinosteroids (BRs) are plant steroid hormones that are essential for normal plant growth and development. Cytochrome P450 monooxygenases play crucial roles in BR biosynthesis, among them, CYP85A families catalyze the last step in the BRs biosynthesis and yield brassinolide (BL) which is the most bioactive BR. It has been shown that CYP85A1 from Arabidopsis thaliana and tomato convert 6-deoxocastasterone (6-deoxoCS) to castasterone (CS), while A. thaliana CYP85A2 and tomato CYP85A3 convert 6-deoxoCS to BL. Although tomato CYP85A1 and CYP85A3 show 85% sequence similarity at the amino acid level, reactions catalyzed by them are different. In order to identify amino acid residues which are crucial for Baeyer-Villiger oxidation involved in BL biosynthesis, we have carried out chimeric studies between tomato CYP85A1 and A3.
植物化学調節学会の論文
2007-10-05