67.LC-ESI-MS/MSによるジャスモン酸誘導体一斉分析法の確立(口頭発表)

概要

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Jasmonic acid (JA) is a plant hormone that has critical roles in plant growth and defense mechanism. JA methyl ester (JA-Me), JA-isoleucine (JA-Ile) and JA-leucine (JA-Leu) have also been proposed to be active forms of JA. To elucidate physiological roles of each JA-derivative in plants, we established a method for quantitative analysis of JA, JA-Me, JA-Ile and JA-Leu by LC-ESI-MS/MS. 100mg FW of Arabidopsis leaves were used as a plant material. JA, JA-Ile and JA-Leu were purified by reverse-phase and anion exchange cartridge columns, and analyzed by LC-ESI-MS/MS. Tuberonic acid, indole-3-acetic acid, abscisic acid and 12-oxo-phytodienoic acid were also quantified in the same analyte. We have developed a new method to analyze JA-Me. JA-Me was immediately derivatized with 2-hydrazino-1-methylpyridine (HMP) after extraction to give a non-volatile derivative, HMP-JA-Me. 100mgFW of Arabidopsis leaves were extracted and derivatized. After purification with a reverse phase column, we were able to quantify HMP-JA-Me by LC-ESI-MS/MS. For quantitative analysis, we prepared stable isotope-labeled JA-Ile and JA-Leu. (+) and (-)-JA-L-amino acid were separated by ODS HPLC and used as internal standards. In this presentation, we will report the existence and levels of each endogenous amino acid conjugate in various plants.
植物化学調節学会の論文
2008-10-06