49.LC-ESI-MS/MSによるブラシノステロイド分析法の構築(口頭発表)

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Brassinosteroids are signal compounds that are involved in the regulation of plant growth and response to environmental stimuli. To analyze endogenous brassinosteroids, gas chromatography-electron impact ionization-mass spectrometry (GC-EI-MS) has been frequently used. An analytical method for castasterone and brassinolide, known as bioactive brassinosteroids, by liquid chromatography-electronspray inonization-tandem mass spectrometry (LC-ESI-MS/MS) has been reported. In this system the detection limit of these compounds were lower than that in common GC-EI-MS systems. We have previously reported a comprehensive analysis method of bioactive hormones such as auxin (indole-3-acetic acid), cytokinins (trans-zeatin and isopentenyladenine), gibberellins, abscisic acid, jasmonates and salicylic acid by LC-ESI-MS/MS. In addition to these compounds, castasterone and brassinolide were analyzed. We first optimized the ionization condition for castasterone and brassinolide using authentic compounds. We found that negative ion mode showed stable intensity of [M-H]- and this molecular ion gave 3 specific product ions that can be used for quantification. By using this optimized condition, a purified sample from 500mg fresh weight of aerial of 4 week old Arabidopsis plants gave 3 specific product ions of castasterone, and the ratio of intensity of these ions were consistent with those of authentic castasterone. In this meeting, we will report the results of other plant materials.
2009-10-06

49.LC-ESI-MS/MSによるブラシノステロイド分析法の構築(口頭発表)

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