5 インゲンのジベレリン3β水酸化酵素の機能と発現制御

概要

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Three genes for gibberellin 3β-hydroxylase (Pv3βOH-1, 2, 3) has been cloned from bean (Phaseolus vulgaris). The deduced amino acid sequence of Pv3βOH-3 has relatively low homology with the 3β-hydrowylase genes already reported, while that of Pv3βOH-2 has high homology with Le gene from pea. Recombinant protein of Pv3βOH-3 catalyzed not only 3β-hydroxylation but also 2, 3-desaturation and epoxydation, and that of Pv3βOH-2 catalyzed only 3β-hydroxylation. Pv3BOH-3 was suggested to be the major 3B-hydroxylase gene expressed in immature seeds, while Pv3βOH-2 was expressed in young leaf.
植物化学調節学会の論文
1999-10-01