P-68 動植物個体均一安定同位体標識化による生細胞・抽出物の多次元NMR計測技術高度化の試み(ポスター発表の部)

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Novel methods for measurement of living systems are making new breakthroughs in life science. As any biological scientist does not doubt importance of metabomics in post-genomics-proteomics era, recent methodological advances of MS-based analysis lead in this field, whereas an NMR-based approaches are recognized as a minor one due to its low sensitivity. Advances in technology for MS have enabled the investigation of cellular proteins and metabolites on a previously unpredicted scale; over 200,000 different compounds will be detected in the plant kingdom. Many of those substances have a potential for the development of synthetic materials and for applications in health and food technology. Thus, plant metabolomics represents a considerable challenge which holds great promise. However, we are trying to initiate this field by developing new methods using a hetero-nuclear NMR-based approach. This has been achieved by the combination of the uniform stable-isotope labeling of higher plants and multi-dimensional NMR experiments [1,2] used in protein structure determination. Using this novel approach, we can analyze the dynamic molecular networks inside cells, tissues and organisms. Furthermore, we have achieved uniform stable-isotope labeling of animals by feeding with uniformly labeled (such as ^<13>C, ^<15>N) plants. Therefore, we propose our methodology; the hetero-nuclear NMR experiments with the uniform stable-isotope labeling would be applicable for metabomics studies in drug discovery, toxicology and health-care assessment.
天然有機化合物討論会の論文
2005-09-15

P-68 動植物個体均一安定同位体標識化による生細胞・抽出物の多次元NMR計測技術高度化の試み(ポスター発表の部)

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