46.シロイヌナズナ種子発芽の高温阻害におけるABAとGA作用の調節(口頭発表)

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Suppression of seed germination by environmental high temperature (thermoinhibition) during summer is crucial for Arabidopsis to establish vegetative growth in autumn. Alleviation of thermoinhibition by an ABA biosynthesis inhibitor, fluridone, and by exogenous GA_3 indicated that stimulation of ABA synthesis and suppression of GA action are critical for germination inhibition. GC/MS analysis showed that high temperature elevated ABA level and suppressed the increase of bioactive GA level in the imbibed seeds. Quantitative real time-PCR analysis revealed that expressions of ABA biosynthesis genes, ZEP, NCED2, NCED5 and NCED9 are up-regulated, and GA biosynthesis genes GA20ox1, GA20ox2, GA20ox3, GABox1 and GA3ox2 are down-regulated by high temperature.These results demonstrate that high temperature stimulates ABA action by enhancing expressions of ABA biosynthesis genes and represses GA action by suppressing expressions of GA synthesis genes in Arabidopsis seeds. Furthermore, in ABA deficient mutant, aba2, the expression of GA3ox genes were increased at high temperature condition. These results indicated that suppression of GA synthesis genes expression was mediated by ABA action. The seeds of loss-of-function mutants of GA negative regulators, SPINDLY(SPY) showed thermoinhibition tolerance, and expression of SPY was up-regulated significantly by high temperature. These results demonstrate that high temperature stimulates GA action by stimulating SPY gene in Arabidopsis seeds.
植物化学調節学会の論文
2007-10-05

46.シロイヌナズナ種子発芽の高温阻害におけるABAとGA作用の調節(口頭発表)

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