18. 植物におけるジテルペン炭化水素の気相への放出とその受容

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ent-Kaurene is a tetracyclic hydrocarbon precursor for gibberellins (GAs) in plants and fungi. In plants, GGDP is converted to the Arabidopsis-kaurene by two separate cyclases, ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS). In contrast, fungal ent-kaurene synthase (GfCPS/KS) converts by bifunctional activities with both CPS and KS. Previously we reported the following results; 1) When co-cultivated with the GfCPS/KS transgenic lines in a closed environment, the dwarf phenotype of ga1 and ga2 mutants was able to fully complement. 2) Co-cultivation of the ga3 mutant, which accumulate ent-kaurene, or of a filter paper containing ent-kaurene also rescued the dwarf phenotype of the mutants. 3) ent-Kaurene was identified from the headspace of the lines and ga3 mutant by GC-MS analysis. These results suggest that ent-kaurene might be metabolized into bioactive GAs in Arabidopsis when supplied as a volatile. To address whether structural differences in the diterpene hydrocarbon affect the emission into the headspace, we generated transgenic Arabidopsis plants overexpressiong a novel fungal diterpene cyclase. The hydrocarbon produced by the enzyme was detected in the headspace of the transgenic lines by GC-MS analysis. This result indicates that Arabidopsis plant is able to release heterologous diterpene to the air. We are currently investigating the effect of the heterologous diterpene on the emission and perception of ent-kaurene by Arabidopsis plants.
植物化学調節学会の論文
2005-10-13

18. 植物におけるジテルペン炭化水素の気相への放出とその受容

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