21. イネのジテルペン型ファイトアレキシン生合成に関するジテルペン環化酵素遺伝子の発現制御機構

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In suspension-cultured rice cells, four types of diterpenoid phytoalexins, momilactones A, B, oryzalexin S, (-)-phytocassans A-E and oryzalexins A-F, are produced in response to treatment with the chitin elicitor N-acetylchitooctaose. Until now, we isolated and identified six diterpene cyclase genes involved in biosynthesis of diterpenoid phytoalexins (OsCyc1, 2, OsDTC1, 2 and OsKS4, 10). We report identification of cis-acting elements responsible for the elicitor-inducible expression of two diterpene cyclase genes, OsDTC2 and OsKS4, in rice cultured cells. OsDTC2 and OsKS4 encode stemar-13-ene synthase and 9βH-pimara-7, 15-diene synthase that are involved in biosynthesis of oryzalexin S and momilactones A, B, respectively. To identify cis-acting elements responsible for chitin elicitor-inducible expression of OsDTC2, the OsDTC2 promoter was analyzed using dual luciferase assay in suspension-cultured rice cells. Deletion and mutation analysis revealed that six W-box motifs between -1634 and -1549 from the translational start site are responsible for the elicitor-inducible expression of OsDTC2. Using the similar methodology, the OsKS4 promoter was analyzed. The results suggest that a TGACGT motif between -1224 and -991 is responsible for the elicitor-inducible expression of OsKS4. The above results suggest that WRKY and bZIP transcriptional factors are involved in elicitor-inducible expression of OsDTC2 and OsKS4, respectively.
植物化学調節学会の論文
2005-10-13

21. イネのジテルペン型ファイトアレキシン生合成に関するジテルペン環化酵素遺伝子の発現制御機構

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