60.イネのジテルペン型ファイトアレキシン生産を制御するbZIP型転写因子OsTGAP1の標的遺伝子の探索(口頭発表)

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Momilactones and phytocassanes are major diterpenoid phytoalexins, which are produced in elicitor-treated rice cells. We recently showed that biosynthetic genes for momilactones are clustered on rice chromosome 4, and that those for phytocassanes are clustered on rice chromosome 2. Moreover, we reported that an elicitor-inducible bZIP transcription factor, OsTGAP1, is involved in the regulation of the expression of biosynthetic genes for diterpenoid phytoalexins. Here we generated and characterized transgenic rice cells constitutively expressing OsTGAP1, and performed microarray analysis using these rice cells. OsTGAP1 over expressing cells exhibited enhanced expression of phytoalexin biosynthetic genes such as OsKSL4 and hyperaccumulation of diterpenoid phytoalexins in response to the chitin elicitor. Next, we performed microarray analysis to identify genes up-regulated in OsTGAP1 over-expressing cells compared to wild-type cells 0, 6, and 24h after elicitor treatment. As a result, 524 genes were up-regulated more than 2-fold in OsTGAP1 over-expressing cells compared to wild-type cells without elicitor treatment. Moreover, 489 genes were up-regulated 6h after elicitor treatment and 335 genes were up-regulated 24h after elicitor treatment. Now, we are performing statistical analysis of these microarray data and a search for the OsTGAP1-binding sites in the promoter regions of OsTGAP1 target genes by ChIP analysis.
2010-10-01