79.イネのエリシター応答性WRKY型転写因子OsWRKY53の標的遺伝子の同定(口頭発表)

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WRKY proteins are a large family of plant-specific transcription factors, and they have been suggested to have a regulatory function in the response to pathogen infection and other stresses. OsWRKY53 is a rice WRKY gene identified by microarray analysis as a chitin oligosaccharide elicitor-induced gene. Previously we indicated that OsWRKY53 functions as a transcriptional activator, and overexpression of OsWRKY53 resulted in enhanced resistance to the rice blast fungus Magnaporthe oryzae in rice plants. To identify direct target genes of OsWRKY53, we generated transgenic rice cells which constitutively express an OsWRKY53-3xHA fusion protein, and established an experimental system for chromatin immunoprecipitation (ChIP). Next, we performed ChIP-on-chip analysis using a custom promoter array which covers promoter regions of chitin oligosaccharide elicitor-induced genes in rice. As the result of ChIP-on-chip analysis, we identified several genes as the candidates of the direct target genes of OsWRKY53. These genes included transcription factors and PR protein genes. Moreover, we performed transcriptome analysis using rice cells overexpressing a dominant-nagative OsWRKY53 mutant. As the result, the expression levels of the several candidate genes decreased in the transgenic rice cells treated with elicitor compared with the similarly treated wild-type rice cells. These results strongly suggest that these selected genes are the direct target genes of OsWRKY53.
植物化学調節学会の論文
2009-10-06

79.イネのエリシター応答性WRKY型転写因子OsWRKY53の標的遺伝子の同定(口頭発表)

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