8 ジャスモン酸結合タンパク質単離に向けたプローブの調製と生理活性

概要

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It has been shown that jasmonic acid (JA) plays important roles in production of secondary metabolites in suspension-cultured cells of rice, soybean, and many other plants. The aim of this study is to prepare chemical probes that are useful for isolation of JA-bindign proteins from the plant cells such as rice and soybean. A dexamethasone-JA conjugate (DEX-JA) that is used for yeast three-hybrid system was prepared by the active ester method, and its biological activity was determined by phenylalanine ammonialydse (PAL)-inducing assay using suspension-cultured soybean cells and the micro-drop method using rice seedlings. DEX-JA was ca. 1/9 as active as JA in the PAL-inducing assay. In the rice cell seedling assay, DEX-JA exhihibited inhibitory effect on the elongation growth in rice seedling, the activity being 1/100 - 1/10 as active as JA. Preparation of JA-amino acid conjugates (JA-alanine, JA-leucine, JA-valine) and fluorescence-labeled JA analogues that are useful for monitoring and analysis of JA-binding proteins is also attempted by the active ester method. The three JA-amino acid conjugateds had almost same activities as JA in both assays. Thus it was proved that DEX-JA and the JA-amino acid conjugates functioned as JA analogues in the plants. Preparation of fluorescence-labeled JA analogues is now under way.
植物化学調節学会の論文
2000-11-02