33 アラビドプシスfackel変異体はステロールC-14還元過程が阻害されているブラシノステロイド欠損株である

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We report here a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons, multiple shoot meristems, and stunted roots. FACKEL gene was found to encode a protein with sequence similarity to both the human sterol reductase family and yeast C-14 reductase. The analyses of sterol compositions in the wild-type and fk-J79 seedlings revealed that fk-J79 mutant accumulated ca. 10 times higher levels of 4α-methyl-5α-ergosta-8,14,24(28)-triene-3β-ol, the substrate of C-14 sterol reductase, and more interestingly, the mutant accumulated high levels of several 8,14-diene sterols. Futhermore, the levels of brassinosteroids, campesterol and campestanol were found to be reduced in the mutant. Unlike other brassinosteroid-deficient mutants, the phenotype was not rescued by the application of brassinosteroids. These results indicate the importance of sterols other than brassinosteroids in plant growth and development.
2000-11-02