26.ジベレリン受容体解析プローブの創製 : in silico screeningによるGID1結合化合物の探索と合成(口頭発表)

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Introduction Gibberellin (GA) is one of the phytohormones that promotes seed germination, stem elongation and flower development. GID1 was identified as GA receptor in 2005. We selected GID1 binding compounds by in silico screening and evaluated their binding activity to GID1 to develop a molecular probe for the analysis of GA-GID1 interaction. Methods and Results We used Surflex docking program for the analysis of the interaction between known GAs and GID1 receptor. Then we selected potent compounds from chemical library data set by in silico virtual screening and evaluated their in vitro binding activity to GID1 with tritium labeled GA. Finally we found a variety of compounds that bind to GID1. The structure-activity relationship study was performed to improve their in vivo and in vitro activity.
2009-10-06