1.オーキシン受容体TIR1に特異的な新規オーキシンプローブの設計と生理活性(口頭発表)

概要

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Arabidopsis F-box protein TIR1 function as an auxin receptor. TIR1, a component of SCF-class E3 ubiquitin-ligase, is responsible for the regulation of the 26S proteasome-mediated destruction of the Aux/IAA family of transcriptional repressers, thereby altering the expression of hundreds of genes. We have designed a series of a novel probes for auxin signaling. BH-IAA is the most potent anti-auxin probe and antagonized all known genomic auxin responses at the molecular and whole-plant level, such as auxin-responsive gene expression, auxin-induced Aux-IAA degradation, auxin-promoted interaction of Aux/IAA-TIRl and physiological auxin responses of Arabidopsis plant. To test the utility of these probes for the analysis of TIR1-mediated auxin signaling in species other than Arabidopsis, we examined the effect of BH-IAA on development in the monocot Oryza saliva (rice) and the lower plant Physcomitrella patens (moss). In both cases, BH-IAA antagonized axuin responses of plants, suggesting that BH-IAA is a useful tool to probe auxin signaling in a variety of plant species. Further, BH-IAA anti-auxin activity in moss demonstrates that P. patens SCF^<TIR1> pathway plays a crucial role in moss development, suggesting SCF^<TIR1> pathway is an ancient mechanism. Our auxin probe provides a new useful tool for plant chemical biology.
植物化学調節学会の論文
2007-10-05