29 放線菌 F-40 株の生産する新規オーキシン信号伝達阻害剤 Terfestatin A

概要

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Bio-probes that modulate the action of auxin are useful tools for the study on auxin signaling. In the screening system for auxin signaling modulators using Arabidopsis reporter line harboring an auxin-inducible promoter comprised of two auxin-responsive elements from Aux/IAA and a glucronidase as reporter gene. Terfestatin A (TrfA) was isolated as a specific inhibitor of auxin signaling from Actinomyces strain F-40. TrfA inhibited the expression of early auxin-responsive genes, such as Aux/IAA (IAA1,5) and SAUR-AC1 genes in arabidopsis. In addition, TrfA was antagonistic to auxin in the expression of early responsive genes, and TrfA itself did not induce these auxin-responsive genes. TrfA also displayed competitive effects on cell elongation and division caused by auxin. On the other hand, auxin inducible GST promoter (perB) was not affected by TrfA. Furthermore, TrfA showed no effects on the expression of other plant hormones, such as GA, ABA and cytokinin, inducible genes. From the above data, TrfA would be specific antagonist on auxin action that lead to early auxin-responsive gene expression, suggesting TrfA might bind auxin receptor in competition with auxin.
植物化学調節学会の論文
2002-10-31