6.細胞内の安定性が高い新規MNI-ケージドオーキシンの合成と生理活性(口頭発表)

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Auxin is polar transported by the transporter proteins, AUX1, PINs and ABCBs, and the transport system establishes an auxin gradient to regulate proper plant development. Thus, the spatial and temporal effect of the auxin gradient on plant development is highly important for the understanding of plant auxin responses. The caging group, 2-nitrobenzyl group was found to be unstable in plant cells and released original auxin without photolysis due to the enzymatic hydrolysis by esterases. Thus, three auxins, indole 3-acetic acid, naphthalene 1-acetic acid and 2,4-dichlorophenoxy acetic acid were caged with the MNI (4-methoxy-7-nitroindolinyl) caging group to form an amide bond between the sec-amino group of MNI group and the carboxylic acid in auxins. MNI-caged auxins, therefore, were very stable within a plant cell, but release auxins with photo-irradiation to activate auxin-responsive reporter gene expression. We evaluated a spatial and temporal control of intracellular auxin levels by photo-irradiation using MNI-caged auxin system, demonstrating that the photo-control of the physiological auxin response in Arabidopsis plants.
2010-10-01