118(P-72) 放線菌F-40株の生産する新規オーキシン信号伝達阻害剤Terfestatin A(ポスター発表の部)

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The plant hormone, auxin, regulates various aspects of plant growth and development by controlling cell division, elongation, and differentiation. However, the molecular mechanism of auxin action is still unclear. Therefore, bio-probes that modulate auxin action are useful tools for study on auxin signaling. In the screening program to search for auxin signaling inhibitors by mean of transgenic Arabidopsis plant harboring early auxin-responsive promoter and reporter gene, GUS (β-glucuronidase), we found terfestatin A (trfA) as a specific inhibitor of auxin signaling from culture bloth of Streptomyces sp. F-40. The chemical structure of trfA was determined to be p-terphenyl-β-glucoside on the basis of various NMR techniques and chemical degradation. TrfA competitively inhibited the expression of early auxin-responsive Aux/IAA genes and also displayed competitive effects on auxin-induced cell division and elongation in Arabidopsis. In contrast to auxin action, trfA showed no effects on the gene expression induced by other plant hormone, such as abscisic acid, gibberellin, and cytokinin. From these results, trfA would be specific antagonist on auxin action that lead to early auxin-responsive gene expression, suggesting trfA might bind unknown auxin receptor in competitive manner.
天然有機化合物討論会の論文
2003-09-01