8.難発根樹種Eucalyptus globulusを用いた発根促進剤のスクリーニング(口頭発表)

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Eucalyptus globulus that is one of the major afforestation species is known to be difficult for vegetative-propagation. Therefore, it was necessary to develop novel method for promotion of adventitious root (AR) formation in E. globulus. First, we measured endogenous hormones at basal part of stem of easy-rooting line and poor-rooting line by UPLC-ESI-qMS/MS. As a result, the Indole-3-acetic acid (IAA) level of easy-rooting line was two times higher than that of the poor-rooting line, suggesting that endogenous IAA level may regulate ability of AR formation. Next, we forcused on the cytochrome P450s that are involved in a vast array of reactions of many different metabolic pathways. A chemical library of triazole derivatives to find chemicals which have the effect of promoting AR formation was screened. Consequently, five compounds effectively promoted AR formation. Finally, we investigated how these chemicals affected the growth of Arabidopsis thaliana. Arabidopsis seedlings were grown on agar medium containing chemicals. One of the selected chemicals, MA65 increased the number of roots in wild-type Arabidopsis seedlings and this phenotype was similar to a mutant superroot2 (sur2). The amounts of endogenous IAA in 14-d-old Arabidopsis grown in the presence of MA65 were analyzed. The IAA content was increased two-fold in the presence of MA65 as compared with untreated. Taken together, MA65 may increase endogenous IAA level in a plant cell and promotes an AR formation.
2010-10-01

8.難発根樹種Eucalyptus globulusを用いた発根促進剤のスクリーニング(口頭発表)

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