28. ストリゴラクトン生合成阻害剤を用いた変異体の探索(口頭発表,植物化学調節学会第46回大会)

概要

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Strigolatones(SLs) were identified from plant root exudates as a germination stimulants for root parasitic weeds which cause severe damage to agriculture in Africa and in the Mediterranean. Arbuscular mycorrhizal (AM) fungi forms symbiosis with many land plants, and SLs also have hyphal branching activity of AM fungi. Recently it was reported that SLs are novel class of phytohormone that inhibit shoot branching, and therefore active studies on the function of SLs in several plants are in progress. However many genes involved in SL biosynthesis or signaling pathway have remained unknown. In the previous work we discovered triazol-type inhibitor of SLs (TIS13). TIS13 reduces endogenous levels of one of major SLs in rice, 2'-epi-5-deoxystrigol, and induce 2nd tiller bud outgrowth. As specific inhibitors are very useful for identifying new genes involved in the function of a biological substance inhibited by the inhibitor, we started to explore SL-related genes by using TIS13 for the screening of new mutants resistant to the function of TIS13. In our mutant screening we used Full-length cDNA over-expressing line (FOX) library, aiming at characterizing genes that cannot be identified from knockout lines because of their functional redundancy. In addition, FOX library easily enables us to identify the genes causing the TIS13 resistance. Here we report the result of the screening of rice FOX library on the basis of the phenotype resistant to TIS13.
2011-10-03