17. ブラシノステロイド情報伝達突然変異体bil5原因遺伝子の機能解析と情報伝達因子特異的阻害剤のケミカルスクリーニングの試行(口頭発表,植物化学調節学会第46回大会)

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Brz (brassinazole) was synthesized as the first specific inhibitor of brassinosteroid biosynthesis. Target of Brz220 is the cytochrome P450 enzyme encoded by DWF4. In order to analyze in detail the mechanisms of brassinosteroid signal transduction, we tried to perform the chemical genetics screening by Brz (brassinazole). Brz caused shorten hypocotyl and opening cotyledon to germination of wild type that was called as photomorphogenesis in the dark. We screened for mutants that showed longer hypocotyls than wild type when grown with Brz220 in the dark, and designated bil mutants (Brz-insensitive-long hypocotyl). We identified a semidominant mutant, bil5, from fast neutron-treated lines. Hypocotyl elongation of these plants on Brz medium was at least twice that of the wild type. Adult bil5 plants showed pale green and thin leaves, and thin and shortened inflorescences. By microscope observation, the palisade cell and spongy cell of bil5 mesophyll tissues were smaller and disturbed in comparison with wild type. Number and size of secondary xylem cell of bil5 inflorescences were decreased. The root elongation of bil5 was also inhibited. Furthermore, current trial for chemical screening targeted to brassinosteroid signalling will be presented at this meeting.
2011-10-03