52.ブラシノステロイド情報伝達突然変異体bss1の原因遺伝子の機能解析(口頭発表)

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We had identified brassinosteroid biosynthesis inhibitor Brz insensitive mutant bill from EMS-mutation lines, and bil5 from fast neutron-mutation lines. To identify additional components of the brassinosteroid signaling, trial to screen new mutants as Brz-insesitive-long hypocotyls in the dark was applied to Arabidopsis activation-tag lines. The semidominant mutant bss1 (Brz-sensitive-short1) showed normally etiolated hypocotyl same as wild type without Brz in the dark. However, with Brz, bss1 hypocotyl became about 50% length of wild type in the dark. In the weak light, hyocotyl of bss1 showed about 50% length of wild type without Brz. These results suggested that bss1 hypocotyl is hypersensitive to Brz and the light. In the later growth stage, bss1 showed extremely dwarf phenotype by inhibition of axis elongation. An activation tag insertion was identified in non-ORF region on bss1 genome. Further analysis is in progress.
2009-10-06