37.ブラシノステロイド情報伝達突然変異体bil2,bil5サプレッサーの解析(口頭発表)

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We had identified brassinosteroid biosynthesis inhibitor Brz insensitive mutant bil1 from EMS-mutation lines, and bil5 from fast neutron-mutation lines. To identify additional components of the brassinosteroid signaling, we screened Arabidopsis activation-tag lines and isolated bil2 that showed long hypocotyls on the medium containing Brz in the dark. Light-grown bil2 mutants had long-petiole phenotype similar to wild-type plants treated with BR or BRI1-ox mutants. By genetic analysis, the candidate gene of bil2 was isolated and the gene encoded a novel cytosolic protein. Identification of overexpress of the bil2 candidate gene is progress. We are investigating semidominant mutants bil5 that mutations were induced to Arabidopsis thaliana Columbia (Col) by Fast neutron. When bil5 was backcrossed with wild type Columbia and the F_2 generation was screened by Brz, obtained all mutants, which hypocotyls showed longer than wild type, grow slender-dwarf phenotype in adult stage. When bil5 was backcrossed with wild type Landsberg erecta (Ler), hypocotyls elongated F_2 generations on Brz segregated to slender dwarf and mild type like phenotype in adult stage. Their results might suggest that a locus of Ler have a function as suppressor of bil5 in adult stage in comparison with Col. Based on the possible difference of species, we tried to identify a suppressor locus for the bil5.
植物化学調節学会の論文
2008-10-06