38.酵母におけるPQQ生合成遺伝子の探索(口頭発表)

概要

論文の詳細を見る
Pyrroloquinoline quinone (PQQ) had been discovered as a coenzyme for dehydrogenase in bacteria, and recognized as a new vitamin in mammals in 2003. Recently, we detected the PQQ from germ-free plants and yeasts with LC-MS/MS. To know the function of PQQ in plants and yeasts, we treated plants and yeasts with PQQ and found that PQQ confers salt stress resistance to both plants and yeasts. These result suggests that plants and yeasts should have PQQ biosynthesis pathway and use it for their survival in various circumstances. So, first we tried to identify the genes that are committed to PQQ biosynthetic pathway in yeasts by screening mutants from yeast deletion lines. Here we reported some of mutants which are less tolerant to salt stress than wild type and their intolerance to salt stress was recovered by the application of PQQ.
2007-10-05