35. イネ幼苗における GA, BR 応答性 Thionin 遺伝子の解析

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Gibberellins (GAs) and brassinosteroids (BRs) play important roles in plant growth and development including stem elongation. GA and BR signal transductions have been well characterized, but little is known about the control mechanisms of gene expression that induce diverse physiological phenomena. We investigated genes controlled by the interaction between GA and BR in rice seedlings by cDNA microarray, concluding that thionin genes were most striking GA/BR-responsive genes. Although rice thionin genes are constitutively expressed in coleoptile, they do not confer resistance to Burkholderia, a major seed-transmitted phytopathogenic bacterium. However, the contribution of rice thionins to resistance to other bacteria is largely unknown. We analyzed the detail expression patterns of rice thionins by Northern-blot analysis and RT-PCR. These analyses suggested that rice thionin mRNA level in coleoptile rapidly decreased in 3 days after germination, and that was suppressed by GA/BR treatment. Furthermore, BR functioned more effectively than GA, and simultaneous treatment showed synergistic effect. Under dark condition, rice thionin gene expression in coleoptile also decreased gradually, but it was kept at higher level than under light condition. These results indicate that the thionin gene expression in rice coleoptile can be a novel monitoring system to study function of plant hormones. This could contribute to elucidate the regulatory gene expression mechanism in response to GA/BR interaction, the enhanced resistance to pathogens by BR, and the relationship between photomorphogenesis and plant hormones.
植物化学調節学会の論文
2003-10-10

35. イネ幼苗における GA, BR 応答性 Thionin 遺伝子の解析

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