28.イムノモジュレーションにより見出されたフィラメント状小胞体の応用(口頭発表)

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We have been working on regulating plant growth by using antibodies, and have succeeded in creating transgenic plants producing single-chain variable fragments (scFvs) against bioactive gibberellins as fusions with GFP, in endoplasmic reticulum (ER). In this study, we used two anti-GA_4 scFv designated as 8/E9 and 21/D13, and in only 21/D13 lines, we found filamentous shape of fluorescence in the leaf epidermal cells. The structure was considered to be an ER-derived compartment, and therefore we here tentatively call it filamentous ER (f-ER). In this study, we examined the applicational possibility of f-ER to produce substances by using <Arabidopsic thaliana>___- transgenic lines. By Western and ELISA analyses, we found that GFP-21/D13 scFv lines keep enormous amount of bioactive GFP-scFv compared to GFP-8/E9 scFv. This result suggested that it could be possible to produce large amount of any antibody in plants by using this structure. We crossed the transgenic lines producing anti-chlorpropham (CIPC) scFv with a GFP-21/D13 scFv line, and analyzed the amount of anti-CPIC scFv when two scFvs were coexpressed. Although we expected that anti-CIPC scFv production level would increase by the filamentous structure, both scFvs production level decreased compared with their original lines, presumably due to decreased gene expression by cosuppression. We are currently analyzing the gene expression level and also trying to produce anti-CPIC scFv as fusion protein with GFP-21/D13 scFv.
2009-10-06

28.イムノモジュレーションにより見出されたフィラメント状小胞体の応用(口頭発表)

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