9. ジベレリンのペプチド性ミミックの同定

概要

論文の詳細を見る
Phage display is a selection technique by which foreign peptides or proteins are expressed as a fusion with coat proteins of a bacteriophage resulting in display of the fused protein on the surface of the virion, while the nucleotide sequence encoding the fusion resides within the virion. Phage display has been used to create a physical link between a library of random peptide sequences to the DNA encoding each sequences, allowing rapid identification of peptide ligands against specific target molecules by a selection technique called panning. We screened a phage display peptide library for peptidyl mimotopes of GA_4 against antibioactive gibberellin antibody, 8E9. The obtained peptides were grouped into two homologous sequences and their binding to the antibody was competed with free GA_4 but not with GA_4 methylester, which is not reactive with 8E9, suggesting that the peptides behave as mimics of GA_4. As an example of application of these peptides, we examined the possibility if these phages can be used as a gibberellin tracer. As a result, the amount of GA_4 from 0.19 to 1.5ng/well could be estimated as a competitor of the phage. This data supports the idea that these phages can be used as a tracer for ELISA analysis of GA_4. These peptides made it possible for the first time to quantify GA_4 by ELISA using 8E9.
植物化学調節学会の論文
2004-10-13