17.イムノモジュレーションにより見出されたフィラメント状小胞体の解析(口頭発表)

概要

論文の詳細を見る
We have previously expressed single-chain variable fragments (scFvs) against bioactive gibberellin (GA), designated as 8/E9 and 21/D13, as fusions with GFP in plants with subcellular localization. In the study, GFP-scFv was most stably accumulated when targeted to endoplasmic reticulum (ER), and the transgenic plants producing GFP-scFv in ER showed dwarf phenotype. Although both 8/E9- and 21/D13-scFvs gave the phenotype, we found as-yet-unrecognized filamentous shape of fluorescence in the leaf epidermal cells only of 21/D13 lines. Because the GFP-scFv has an KDEL ER-retention signal, the structure was considered to be an ER-derived compartment, and therefore we here tentatively call it filamentous ER (f-ER). The appearance of f-ER is like an long thread entwining complicatedly. The f-ER is present in the inner part of the cells compared to ER networks locating beneath plasma membranes, and has much stronger fluorescence than ER networks. Interestingly, the filamentous structure was fragmented immediately after treatment with GA4, the ligand of the scFv, suggesting that the structure is induced and maintained by binding of the scFv with an unknown target in the ER. Analysis of this phenomenon might give an insight to the mechanism of ER development.
2007-10-05