69.ブラシノステロイド情報伝達突然変異体bil2,bpgの機能解析(口頭発表)

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We had identified brassinosteroid biosynthesis inhibitor Brz insensitive mutant bil1 from EMS-mutation lines, and bil5 from fast neutron-mutation lines. To identify additional components of the brassinosteroid signaling, trial to screen new mutants as Brz-insensitive-long hypocotyls in the dark was applied to Arabidopsis activation-tag lines. We screened Arabidopsis activation-tag lines and isolated bil1 that showed long hypocotyls on the medium containing Brz in the dark. Light-grown bil2 mutants had long petiole phenotype similar to wild-type plants treated with BR or BRI1-OX mutants. These phenotypes suggested that bil1 mutants enhanced the BR signaling. Identification of overexpression the BIL2 candidate gene is progress. We are starting to sreen new bpg (Brz-insensitive pale green) mutant that showed pale green leaves on the Brz medium in the light. In this study, we used FOX (Full-length cDNA over expresser gene) hunting system lines, which were over expression of a normalized Arabidopsis full-length cDNA library.
2010-10-01