15.ヤリブ試薬による大麦糊粉層ジベレリン情報伝達の阻害に関する研究(口頭発表)

概要

論文の詳細を見る
Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at plasma membrane and extracellular space. A synthetic chemical reagent called β-glucosyl Yariv reagent (β-GlcY) binds specifically with AGPs. We have reported that gibberellin (GA) signaling was specifically inhibited by β-GlcY treatment in barley aleurone protoplasts. In this study, we examined the specificity of inhibitory effect of β-GlcY on GA signaling using microarray analysis and found that β-GlcY was largely effective in repressing both the GA-induced- and repressed-gene expressions. In addition, quite a number of genes were up-regulated by β-GlcY in a GA-independent manner, and many of them were categorized to defense-related genes including two WRKYs and Esi47, which were recently shown to repress GA signaling. Plant defense signaling triggered by several plant defense system inducers such as jasmonic acid or chitin elicitor could inhibit GA-inducible events such as α-amylase secretion, programmed cell death, and gene induction of some GA-inducible genes in aleurone cells. These results indicate that GA signaling in aleurone cells is under regulation of defense-related signaling, which is presumably mediated by WRKYs and Esi47 at least in part. It is also probable that AGPs are involved in the perception of stimuli causing defense responses.
植物化学調節学会の論文
2007-10-05