22. ブラシノステロイド情報伝達突然変異体のFOXラインからの探索と新しい変異体bil7の植物形態形成における機能解析(口頭発表,植物化学調節学会第46回大会)

概要

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We had identified several brassinosteroid signaling mutants from EMS-mutation lines, fast neutron-mutation lines and activation-tag lines in Arabidopsis. To identify new brassinosteroid signaling mutants, we used FOX (Full-length cDNA over-expressor gene) hunting system lines, which were overexpressed mutants of a normalized Arabidopsis full-length cDNA library. For these screening, we used Brz that is an inhibitor of brassinosteroid biosynthesis enzyme DWF4. Brz treatment caused to wild type as shorten hypocotyls in the dark. We tried to screen from about 8000 Fox-lines for bil(Brz-insensitive-long hypocotyls) that showed longer hypocotyls than wild type with Brz in the dark, and identified bil7 mutant. At adult stage, bil7 showed taller inflorescence than wild type. It appears that possible bil7 is brassinosteroid signaling factor and promote inflorescence elongation. Further analyses are in progress.
2011-10-03