13.ジベレリンシグナル伝達制御剤の探索[II] : 受容体機能阻害剤(口頭発表)

概要

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Direct interaction of a gibberellin receptor GID1 with a signal repressor DELLA triggers the transduction of gibberellin signal in plants. The binding of the two molecules occurs only in the presence of gibberellin. In this study, we developed a system for screening of the chemicals which inhibit the GID1-DELLA binding, based on a yeast two-hybrid system. When biologically active gibberellin exists, the yeast can grow even in the undernourished medium. By using its growth response in the co-presence of gibberellin and each examined chemical, we performed a random screening of inhibitors to the interaction between GID1 and DELLA. We found that some chemicals inhibited this binding. Then, we examined their inhibitory effects in vitro and in planta. Finally one chemical which showed inhibitory effects to both Arabidopsis and Barley, was selected.
2010-10-01