24. アサガオ未熟種子に由来する多機能型糖転位酵素の単離と応用

概要

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Glucosylated plant hormones occur in many plant species, and they are supposed to be catabolized forms to maintain the hormones at suitable levels in tissues, or the forms for translocation. However their physiological roles remain unclear. We cloned cDNAs encoding glucosyltransferases (GTases) that catalyze glucosylation of phytohormones from developing seeds of morning glory (Ipomoea nil) to have information on the metabolic dynamics of phytohormones in seed development. After degenerate-PCRs and RACEs using primers for the conserved region in many orthologous GTase genes and using cDNAs prepared from immature seeds at various maturities (3-33 day-after-flowering) of morning glory, we obtained nine full-length cDNAs, prepared GST-fused recombinant proteins using an E. coli expressional system, and studied their enzymatic activities. The recombinants were incubated with gibberellins (GAs), zeatin, indole-3-acetic acid (IAA), abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) in the presence of ^<14>C-UDP-glucose as a sugar donor, and the radioactive metabolites were visualized on imaging plates after TLC. A recombinant, designated InGTase1, showed a GTase activity by converting ABA, IAA, JA, and SA to polar metabolites.
植物化学調節学会の論文
2005-10-13