1 抗GA一本鎖抗体の植物における発現と形質転換体植物の解析

概要

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"Immunomodulation" has been proposed to be a new technology to control plant growth by expressing antibodies against plant growth regulators. We have already succeeded in expressing the functional single-chain variable fragment (scFv) against GA_24, a precursor of bioactive GA (GA_4), in tobacco. Athough stem growth of the transgenic plants appeared to be reduced, the relationship between the phenotype and the accumulation of scFv protein has yet to be clear. To analyze this relation in more detail by using a large number of transgenic plants, we introduced the anti-GA_24 scFv into Arabidopsis. The vetor was designed to target the scFv into ER, using the combination of signal peptide and ER-retention signal (fig.1). The second generation from one transgenic line showed reduced stem growth in comparison with wild type. The detail analysis is in progress. We also prepared a new hybridoma cell line secreting antibioactive GA (GA_4) monoclonal antibody. ScFv gene was prepared by tandem connection of V_H gene, linker gene, and V_L gene, and was cloned into a phagemid vector, pCANTAB5E(fig.2), for phage display. The binding activity of the anti-GA_4 scFv is being examined.
植物化学調節学会の論文
1999-10-01